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Updated: Jun 3, 2026

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
Published on: May 29, 2018
Volume localized shift selective ¹³C spectroscopy using pulsed rotating frame transfer sequences with windows (PRAWN)
Abhishek Banerjee1, N Chandrakumar
1Department of Chemistry, Indian Institute of Technolog-Madras, Chennai, Tamil Nadu, India.
Novel techniques for selective carbon-13 (¹³C) spectroscopy utilize rotating frame J cross-polarization. These methods demonstrate efficient performance with low radiofrequency duty cycles and tolerance to mismatch, enabling selective fat detection.
Area of Science:
- Magnetic Resonance Imaging
- Spectroscopy
- Biophysical Chemistry
Background:
- Chemical shift selective (¹³C) spectroscopy is crucial for molecular analysis.
- Existing techniques face limitations in efficiency and robustness.
Purpose of the Study:
- To introduce and evaluate novel techniques for volume-localized, chemical shift selective (¹³C) spectroscopy.
- To assess the performance of rotating frame J cross-polarization based methods.
Main Methods:
- Development of selective mixing sequences: pulsed rotating frame transfer sequences with windows (PRAWN) and PRAWN-π.
- Utilized two volume localization modules: point resolved spectroscopy (PRESS) and localized distortionless enhancement by polarization transfer (LODEPT).
- Systematic performance evaluation using liquid and gel phantoms.
Main Results:
- Demonstrated efficient operation of J cross-polarization sequences at very low radiofrequency duty cycles.
- Showcased considerable tolerance to Hartmann-Hahn mismatch.
- Successfully performed selective detection of saturated fat in pigeon egg using LODEPT-PRAWN-π in indirect detection mode.
Conclusions:
- The novel techniques offer efficient and robust solutions for selective (¹³C) spectroscopy.
- These methods are suitable for various applications, including in vivo metabolic studies.
- The demonstrated selective fat detection highlights the potential of these techniques in complex biological samples.
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