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Updated: Jun 3, 2026

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

Published on: November 1, 2015

Humanized SCID mice models of SLE.

Nikola Kerekov¹, Nikolina Mihaylova, Jozsef Prechl

¹Department of Immunology, Stefan Angelov Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.

Current Pharmaceutical Design

|April 8, 2011

Summary

This study explores a novel therapy for Systemic Lupus Erythematosus (SLE) by targeting DNA-specific B cells. A new chimeric molecule aims to restore tolerance to DNA in humanized mice, offering a potential treatment for SLE patients.

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Area of Science:

Immunology
Molecular Biology
Therapeutic Development

Background:

Pathological DNA-specific B cells drive Systemic Lupus Erythematosus (SLE).
Complement receptor type 1 (CR1) on lymphocytes exhibits suppressive activity.
Co-crosslinking CR1 with the B-cell receptor (BCR) inhibits B cell activation and proliferation.

Purpose of the Study:

To investigate a novel therapeutic strategy for SLE.
To evaluate the potential of a chimeric molecule to re-establish tolerance to native DNA in humanized mice.

Main Methods:

Utilized humanized severe combined immunodeficiency (SCID) mice engrafted with cells from SLE patients.
Administered a chimeric molecule composed of an antibody against human inhibitory CR1 and a DNA-mimicking decapeptide.

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Assessed the molecule's ability to co-crosslink B-cell receptors and inhibitory surface receptors.

Main Results:

The chimeric molecule demonstrated the capacity to co-crosslink target receptors selectively.
This co-crosslinking delivered a potent suppressive signal to autoreactive B cells.
The approach showed potential for re-establishing tolerance to native DNA.

Conclusions:

A protein-engineered chimeric molecule targeting CR1 and BCR offers a promising therapeutic avenue for SLE.
This strategy may induce tolerance to DNA by delivering suppressive signals to pathological B cells.
Humanized SCID mice serve as a valuable model for testing SLE therapies.