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Molecular discrimination of sheep bovine spongiform encephalopathy from scrapie

Laura Pirisinu1, Sergio Migliore, Michele Angelo Di Bari

  • 1Istituto Superiore di Sanita, Rome, Italy.

Emerging Infectious Diseases
|April 8, 2011
PubMed

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Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.