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Updated: Aug 12, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
Published on: August 6, 2019
Conserved domains of human U4 snRNA required for snRNP and spliceosome assembly
1Max-Planck-Institut fuer Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, FRG.
Abstract:
U4 snRNA is phylogenetically highly conserved and organized in several domains. To determine the function of each of the domains of human U4 snRNA in the multi-step process of snRNP and spliceosome assembly, we used reconstitution procedures in combination with snRNA mutagenesis. The highly conserved 5' terminal domain of U4 snRNA consists of the stem I and stem II regions that have been proposed to base pair with U6 snRNA, and the 5' stem-loop structure. We found that each of these structural elements is essential for spliceosome assembly. However, only the stem II region is required for U4-U6 interaction, and none of these elements for Sm protein binding. In contrast, the 3' terminal domain of U4 snRNA containing the Sm binding site is dispensable for both U4-U6 interaction and spliceosome assembly. Our results support an organization of the U4 snRNP into multiple functional domains, each of which acts at distinct stages of snRNP and spliceosome assembly.
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