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Microarray Polymer Profiling (MAPP) for High-Throughput Glycan Analysis
Published on: September 29, 2023
Glycome diagnosis of human induced pluripotent stem cells using lectin microarray
Hiroaki Tateno1, Masashi Toyota, Shigeru Saito
1From the Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.
The Journal of Biological Chemistry
|April 8, 2011
Summary
Reprogramming somatic cells into induced pluripotent stem cells (iPSCs) alters their glycan profiles, making them resemble embryonic stem cells (ESCs). Lectin microarrays can distinguish between pluripotent and differentiated cells, aiding stem cell diagnosis.
Area of Science:
- Stem cell biology
- Glycobiology
- Cellular reprogramming
Background:
- Induced pluripotent stem cells (iPSCs) are generated from somatic cells (SCs) via transcription factor expression.
- Reprogramming induces global changes in gene expression and epigenetics.
- The impact of reprogramming on cellular glycan repertoires remains largely uncharacterized.
Purpose of the Study:
- To comprehensively analyze and compare the glycomes of human iPSCs and ESCs.
- To investigate how transcription factor-induced reprogramming affects the cellular glycan repertoire.
- To identify specific glycan features characteristic of the pluripotent state.
Main Methods:
- Generated 114 types of human iPSCs from five different SC lines.
- Utilized a high-density lectin microarray for comprehensive glycan analysis.
- Compared glycomes of undifferentiated iPSCs/ESCs with differentiated SCs.
- Performed unsupervised cluster analysis and statistical selection of discriminating lectins.
- Correlated lectin microarray results with glycosyltransferase gene expression profiles.
Main Results:
- Undifferentiated iPSCs and ESCs clustered together, distinct from differentiated SCs.
- SCs with distinct initial glycan profiles acquired ESC-like glycomes upon reprogramming.
- Thirty-eight lectins were identified as discriminators between SCs and pluripotent cells.
- The lectin rBC2LCN specifically detected undifferentiated iPSCs/ESCs.
- Glycosyltransferase gene expression correlated well with glycan profiles.
Conclusions:
- Cellular glycome analysis is a valid method for comprehensive glycan profiling.
- Reprogramming significantly alters the cellular glycan repertoire, conferring pluripotency-associated features.
- High-density lectin microarrays are effective tools for stem cell diagnosis based on cellular glycome.
