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Electron capture, collision-induced, and electron capture-collision induced dissociation in Q-TOF
Valery G Voinov1, Max L Deinzer, Joseph S Beckman
1Department of Chemistry, Oregon State University, Corvallis, OR 97331, USA. valery.voinov@orst.edu
Journal of the American Society for Mass Spectrometry
|April 8, 2011
Summary
Electron-capture dissociation (ECD) and collision-induced dissociation (CID) can be performed simultaneously in a novel radio-frequency-free electromagnetostatic cell. This method allows high-resolution mass spectra with flexible control over peptide fragmentation.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Proteomics
Background:
- Radio-frequency-free electromagnetostatic (rf-free EMS) cells enable electron-capture dissociation (ECD) without cooling gas or specific electron injection.
- Previous work demonstrated simultaneous ECD and collision-induced dissociation (CID) in a triple quadrupole mass spectrometer.
Purpose of the Study:
- To demonstrate high-resolution mass spectra acquisition using ECD and CID.
- To showcase flexible control over peptide fragmentation processes.
- To adapt the rf-free EMS cell for use in a hybrid Q-TOF tandem mass spectrometer.
Main Methods:
- Utilized a newly designed rf-free EMS cell integrated into a hybrid Q-TOF tandem mass spectrometer.
- Performed simultaneous electron-capture dissociation (ECD) and collision-induced dissociation (CID) on peptides.
- Recorded high-resolution product-ion mass spectra.
Main Results:
- Achieved high-resolution mass spectra for ECD and CID.
- Demonstrated flexible control over peptide fragmentation.
- Successfully adapted the rf-free EMS cell for a hybrid Q-TOF platform.
Conclusions:
- The newly designed rf-free EMS cell facilitates simultaneous ECD and CID in a hybrid Q-TOF mass spectrometer.
- This approach offers enhanced control over fragmentation, enabling high-resolution analysis of peptide products.
- The findings advance the capabilities of tandem mass spectrometry for complex biological sample analysis.
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