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Updated: Jun 2, 2026
A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
Published on: January 12, 2017
Shotgun proteome expression profile of microdissected superficial transitional cell carcinoma and biomarker discovery
Hai-Tao Niu1, Shu-Juan Li, Ting Xu
1Department of Urology, Affiliated Hospital of the Medical College of Qingdao University, Qingdao 266003, P.R. China. niuhaitao1976@yahoo.com.cn.
Abstract:
The aim of this study was to globally characterize the expression profile of superficial transitional cell carcinoma using shotgun proteome strategy and to discuss the biomarker panel identified from urine. We identified 440 commonly expressed proteins from four samples of superficial transitional cell carcinoma using laser capture microdissection coupled with two-dimensional liquid chromatography tandem mass spectrometry. The identified proteins were further analyzed using bioinformatics tools and compared with the published literature. Compared with the entire list of the International Protein Index, there were 41/22 Gene ontology (GO) terms found to be enriched/depleted within the biological process annotation. GO biological process enrichment/depletion analysis was consistent with the results of urine proteome analysis. Proteins classified under the terms cell adhesion, cell proliferation and cell differentiation are good candidate biomarkers for cancer detection from urine. In conclusion, the present study identified an extensive expression profile in superficial transitional cell carcinoma of the bladder, providing information for the understanding of cancer cell biology and the discovery of a biomarker panel from urine.
Insights
This study characterized bladder cancer protein expression using shotgun proteomics. Researchers identified potential urinary biomarkers for early cancer detection, including proteins involved in cell adhesion and proliferation.
Area of Science:
- Urology
- Proteomics
- Oncology
Background:
- Superficial transitional cell carcinoma (TCC) is the most common type of bladder cancer.
- Accurate diagnostic biomarkers for early detection remain a challenge.
Purpose of the Study:
- To globally characterize the protein expression profile of superficial TCC.
- To identify potential urinary biomarkers for bladder cancer detection.
Main Methods:
- Shotgun proteomic strategy utilizing laser capture microdissection.
- Two-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS).
- Bioinformatics analysis including Gene Ontology (GO) enrichment.
Main Results:
- Identified 440 commonly expressed proteins in superficial TCC samples.
- Bioinformatics analysis revealed enriched/depleted GO terms related to biological processes.
- Proteins involved in cell adhesion, proliferation, and differentiation were highlighted as potential biomarkers.
Conclusions:
- The study provides a comprehensive protein expression profile for superficial TCC.
- Identified proteins offer a promising biomarker panel for non-invasive bladder cancer detection in urine.
