Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

The Movement of Organelles and Vesicles

The Movement of Organelles and Vesicles

In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...

Microtubule Associated Motor Proteins

Microtubule Associated Motor Proteins

Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular cargos...

Directionality of Nuclear Transport

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...

Directing Proteins to the Rough Endoplasmic Reticulum

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Clonal dynamics deviate from neutral drift in zebrafish spermatogenesis.

bioRxiv : the preprint server for biology·2026

Same author

Interferon signaling is required for early neutrophil recruitment after zebrafish heart injury.

Biology open·2026

Same author

PGE2 modulation of stimulus valence directs zebrafish behavioral fever.

bioRxiv : the preprint server for biology·2026

Same author

A fast-to-faithful transition shapes the DNA repair landscape during embryogenesis.

bioRxiv : the preprint server for biology·2026

Same author

Early ultraviolet exposure disrupts late dorsal fin skeletal development in zebrafish.

Developmental dynamics : an official publication of the American Association of Anatomists·2026

Same author

MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryos.

Nature communications·2026

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

Molecular motors: directing traffic during RNA localization.

James A Gagnon¹, Kimberly L Mowry

¹Department of Molecular Biology, Cell Biology & Biochemistry, Brown University, Providence, RI, USA.

Critical Reviews in Biochemistry and Molecular Biology

|April 12, 2011

Summary

RNA localization directs gene expression for cellular polarity. Motor proteins are key to this polarized transport, with recent studies clarifying their complex roles in moving RNA to specific destinations.

More Related Videos

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Related Experiment Videos

Last Updated: Jun 2, 2026

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Published on: October 30, 2014

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Published on: June 27, 2013

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

RNA localization is crucial for establishing cellular polarity and spatially restricting gene expression.
While the outcomes of RNA localization are understood, the precise mechanisms driving polarized transport are still being uncovered.

Purpose of the Study:

To review recent findings on the mechanisms of RNA localization.
To clarify the pathways and roles of molecular motor proteins in directed RNA movement.

Main Methods:

Review of recent scientific literature on RNA localization.
Analysis of studies investigating molecular motor protein involvement in RNA transport.

Main Results:

Molecular motor proteins play significant roles in RNA localization.
Coordinated action of motor proteins guides RNA to specific subcellular locations.
Diverse systems reveal complex, multi-step mechanisms for RNA transport.

Conclusions:

Understanding motor protein function is essential for deciphering RNA localization mechanisms.
Recent research highlights the intricate coordination required for directed RNA movement.
Further investigation into these pathways will illuminate fundamental cellular processes.