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Related Concept Videos

Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
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Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
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In Vitro Analysis of E3 Ubiquitin Ligase Function
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Published on: May 14, 2021

Processive ubiquitin chain formation by the anaphase-promoting complex.

Hermann-Josef Meyer1, Michael Rape

  • 1University of California at Berkeley, Department of Molecular and Cell Biology, Berkeley, CA 94720-3202, United States.

Seminars in Cell & Developmental Biology
|April 12, 2011
PubMed
Summary

The anaphase-promoting complex (APC/C) controls cell division by tagging proteins for destruction. This study explores how APC/C

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Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Progression through mitosis depends on the timely degradation of cell cycle regulators.
  • The anaphase-promoting complex (APC/C) orchestrates this degradation via ubiquitination and proteasomal pathways.
  • APC/C must precisely regulate substrate degradation to ensure accurate cell division.

Purpose of the Study:

  • To elucidate the mechanisms by which the APC/C achieves processive ubiquitin chain formation.
  • To understand how APC/C discriminates between its diverse substrates during mitosis.
  • To explain how ubiquitin chain assembly drives sequential degradation of mitotic regulators.

Main Methods:

  • The abstract does not specify methods.
  • Further details on experimental approaches are needed.

Main Results:

  • APC/C utilizes differences in ubiquitin chain assembly processivity to distinguish substrates.
  • Processive ubiquitin chain formation is key to triggering sequential degradation.
  • This mechanism ensures orderly progression through mitosis.

Conclusions:

  • APC/C's ability to form processive ubiquitin chains is crucial for mitotic progression.
  • Substrate discrimination by APC/C relies on ubiquitin chain assembly dynamics.
  • Understanding these mechanisms provides insights into cell cycle control.