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Updated: Jun 2, 2026

Analysis of Lipid Droplet Content in Fission and Budding Yeasts using Automated Image Processing
Published on: July 17, 2019
The putative Saccharomyces cerevisiae hydrolase Ldh1p is localized to lipid droplets
Sven Thoms1, Mykhaylo O Debelyy, Melanie Connerth
1Institut für Physiologische Chemie, Ruhr-Universität Bochum, Universitätsstr. 150, D-44780 Bochum, Germany.
Abstract:
Here, we report the identification of a novel hydrolase in Saccharomyces cerevisiae. Ldh1p (systematic name, Ybr204cp) comprises the typical GXSXG-type lipase motif of members of the α/β-hydrolase family and shares some features with the peroxisomal lipase Lpx1p. Both proteins carry a putative peroxisomal targeting signal type1 (PTS1) and can be aligned with two regions of homology. While Lpx1p is known as a peroxisomal enzyme, subcellular localization studies revealed that Ldh1p is predominantly localized to lipid droplets, the storage compartment of nonpolar lipids. Ldh1p is not required for the function and biogenesis of peroxisomes, and targeting of Ldh1p to lipid droplets occurs independently of the PTS1 receptor Pex5p.
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