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Label-free Single Molecule Detection Using Microtoroid Optical Resonators
Published on: December 29, 2015
A single-layer, planar, optofluidic Mach-Zehnder interferometer for label-free detection
Michael Ian Lapsley1, I-Kao Chiang, Yue Bing Zheng
1Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA 16802, USA.
Lab on a Chip
|April 12, 2011
Summary
We developed a simple, low-cost optofluidic Mach-Zehnder interferometer for label-free detection. This device offers high sensitivity and low variability, making it ideal for sensitive biosensing applications.
Area of Science:
- Optofluidics
- Interferometry
- Biosensing
Background:
- On-chip interferometers often require complex fabrication.
- Label-free detection of liquid samples is crucial for biosensing.
- Existing optofluidic refractometers can be expensive and difficult to produce.
Purpose of the Study:
- To develop a planar, optofluidic Mach-Zehnder interferometer.
- To achieve label-free detection of liquid samples with high sensitivity.
- To utilize a simple fabrication process and basic optical equipment.
Main Methods:
- Fabrication via single-layer soft lithography.
- Utilized a single-wavelength laser and silicon photodetector for data collection.
- Calibrated using calcium chloride solutions and tested with bovine serum albumin detection.
Main Results:
- Achieved a low limit of detection (1.24 × 10(-4) RIU).
- Demonstrated low variability (1 × 10(-4) RIU) and high sensitivity (927.88 oscillations per RIU).
- Performance comparable to state-of-the-art devices with complex fabrication.
Conclusions:
- The developed device offers a promising, cost-effective solution for label-free optical detection.
- Simple fabrication and straightforward optics enable mass production potential.
- Suitable for developing inexpensive, highly sensitive biosensing systems.
