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The expression of SOX11, cyclin D1, cyclin D2, and cyclin D3 in B-cell lymphocytic proliferative diseases

Xin Cao1, Lei Fan, Cheng Fang

  • 1Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, 210029 Nanjing, China.

Medical Oncology (Northwood, London, England)
|April 12, 2011
PubMed

Insights

SOX11 and cyclin D1 mRNA expression is elevated in mantle cell lymphoma (MCL) compared to other B-cell lymphocytic proliferative diseases (B-LPD). Nuclear SOX11 protein was specifically detected in MCL patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Hematology

Background:

  • SOX11 is involved in neurogenesis and tissue remodeling.
  • D cyclins (cyclin D1, D2, D3) are implicated in cell transformation.
  • The role of SOX11 and D cyclins in B-cell lymphocytic proliferative diseases (B-LPD) requires further clarification.

Purpose of the Study:

  • To assess the expression of SOX11, cyclin D1, cyclin D2, and cyclin D3 mRNA and SOX11 protein in B-LPD.
  • To clarify the clinical significance of these genes in B-LPD.

Main Methods:

  • Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for mRNA expression analysis.
  • Immunohistochemistry for SOX11 protein detection.
  • Analysis of 152 B-LPD patients for mRNA and 15 for protein.

Main Results:

  • SOX11 and cyclin D1 mRNA levels were higher in mantle cell lymphoma (MCL) than in chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), hairy cell leukemia (HCL), splenic marginal zone lymphoma (SMZL), and healthy controls.
  • Cyclin D1 and cyclin D2 mRNA levels were higher in DLBCL than in SMZL.
  • Nuclear SOX11 protein was detected in 3 out of 4 MCL patients, but not in other B-LPDs. ZAP70-positive CLL patients showed higher SOX11 expression than ZAP70-negative patients.

Conclusions:

  • Mantle cell lymphoma (MCL) patients exhibit higher SOX11 and cyclin D1 mRNA expression.
  • Nuclear SOX11 protein expression is a potential biomarker for MCL.
  • SOX11 and D cyclins show significant correlations in B-LPD, suggesting a role in disease pathogenesis.

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