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Juanmin Zha1, Xin Chen, Chaojun Li

  • 1Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China.

Molecular Biotechnology
|April 12, 2011
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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