Related Experiment Video
Updated: Jun 2, 2026

10:25
Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
Published on: December 9, 2011
A method for studying knife tool marks on bone.
Kai-Ping Shaw1, Ju-Hui Chung, Fang-Chun Chung
1Department of Forensic Pathology, Institute of Forensic Medicine, Ministry of Justice, Taipei, Taiwan. kpshaw596@gmail.com
Journal of Forensic Sciences
|April 13, 2011
Summary
This study analyzed knife marks on bone, revealing a correlation between the knife
Area of Science:
- Forensic Science
- Bioengineering
- Materials Science
Background:
- Knife tool marks on hard tissues provide crucial forensic evidence.
- Understanding the relationship between knife characteristics and bone marks aids investigations.
Purpose of the Study:
- To characterize V-shape tool marks on bone resulting from chopping actions.
- To establish a correlation between knife angle, bone elasticity, and mark characteristics.
Main Methods:
- A custom chopping stage with a gravity accelerator was used to simulate knife impacts on pig bone.
- A digital microscope measured the knife angle (θ) and the resulting V-shape tool mark angle (ψ).
- The elasticity coefficient (κ = θ/ψ) was calculated and correlated with impulsive force.
Main Results:
- A consistent correlation was found between the V-shape tool mark angle (ψ) and the elasticity coefficient (κ) under constant impulsive force.
- The study quantified the relationship between the knife's physical attributes and the resulting bone tissue damage.
Conclusions:
- The derived elasticity coefficient (κ) is a valuable metric for analyzing knife marks on bone.
- These findings enhance the medicolegal investigation of sharp force trauma involving knives.

