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Enterococcus faecalis sufCDSUB complements Escherichia coli sufABCDSE.

Gustavo P Riboldi1, Timothy J Larson, Jeverson Frazzon

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This summary is machine-generated.

Iron-sulfur [Fe-S] cluster biogenesis machinery varies between bacterial groups. The Enterococcus faecalis SUF system effectively complements other bacterial iron-sulfur cluster assembly systems, highlighting conserved functional interactions.

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Area of Science:

  • Biochemistry
  • Microbiology
  • Molecular Biology

Background:

  • Iron-sulfur [Fe-S] clusters are vital cofactors in all life forms.
  • Bacterial [Fe-S] cluster biogenesis involves complex protein machinery, with distinct systems (NIF, ISC, SUF) in Proteobacteria and a less-characterized SUF-based system in Firmicutes.
  • Enterococcus faecalis, a clinically relevant Firmicutes, possesses a sufCDSUB [Fe-S] cluster biosynthetic system.

Purpose of the Study:

  • To compare the functional capabilities of Proteobacteria and Firmicutes [Fe-S] biogenesis systems.
  • To investigate the ability of the Enterococcus faecalis sufCDSUB system to complement known [Fe-S] biogenesis pathways in other bacteria.
  • To elucidate similarities and variations in [Fe-S] cluster assembly machinery across different bacterial phyla.

Main Methods:

  • Comparative functional analysis of [Fe-S] biogenesis systems.
  • Complementation assays using Azotobacter vinelandii and Escherichia coli ISC and SUF systems.
  • Genetic manipulation of bacterial strains to assess system functionality.

Main Results:

  • The Enterococcus faecalis sufCDSUB system successfully complemented the Escherichia coli SUF system.
  • Viable mutants were obtained for both sufABCDSE and iscRSU-hscBA-fdx systems when complemented by E. faecalis SUF factors.
  • The study demonstrated that the complete set of E. faecalis SUF factors facilitates proper functional interactions essential for [Fe-S] cluster biogenesis.

Conclusions:

  • The Enterococcus faecalis [Fe-S] cluster biogenesis system exhibits functional compatibility with other bacterial systems.
  • Conserved functional interactions are crucial for the assembly of iron-sulfur clusters, even between proteins from different bacterial phyla.
  • This research provides insights into the evolutionary conservation and functional diversity of [Fe-S] biogenesis pathways in bacteria.