Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
Meiosis II02:02

Meiosis II

Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis II01:57

Meiosis II

Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each containing...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human dynein-dynactin is a fast processive motor in living cells.

eLife·2026
Same author

Grip it and rip it.

Nature chemical biology·2026
Same author

CTCF maintains centromere function and mitotic fidelity.

Journal of cell science·2026
Same author

Proximity-based activation of AURORA A by MPS1 potentiates error correction.

Current biology : CB·2025
Same author

Proximity-based activation of AURORA A by MPS1 potentiates error correction.

bioRxiv : the preprint server for biology·2024
Same author

Multimerization of a disordered kinetochore protein promotes accurate chromosome segregation by localizing a core dynein module.

The Journal of cell biology·2024

Related Experiment Video

Updated: Jun 2, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Chromosome segregation: a kinetochore missing link is found.

Thomas J Maresca1

  • 1Biology Department, University of Massachusetts Amherst, Amherst, MA 01003, USA. tmaresca@bio.umass.edu

Current Biology : CB
|April 13, 2011
PubMed
Summary
This summary is machine-generated.

Researchers identified a key molecular link connecting the inner and outer kinetochore during cell division. This finding clarifies how the kinetochore, crucial for mitosis, connects centromeric chromatin to microtubules.

More Related Videos

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

Related Experiment Videos

Last Updated: Jun 2, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The kinetochore is a complex protein structure that assembles on centromeric chromatin during mitosis.
  • It serves as the crucial interface between chromosomes and spindle microtubules, ensuring accurate chromosome segregation.
  • A conserved molecular linkage connecting the inner (chromatin-associated) and outer (microtubule-binding) kinetochore domains has remained elusive.

Purpose of the Study:

  • To identify the molecular component responsible for linking the inner and outer kinetochore domains.
  • To elucidate the conserved mechanism of kinetochore assembly and function during mitosis.

Main Methods:

  • Comparative genomics and proteomics across diverse species.
  • Biochemical assays to test protein-protein interactions.
  • High-resolution microscopy to visualize kinetochore structure and dynamics.

Main Results:

  • Two independent studies have identified a conserved molecular linkage between the inner and outer kinetochore.
  • This linkage is essential for the proper assembly and function of the kinetochore during cell division.

Conclusions:

  • The identified molecular linkage provides a critical missing piece in understanding kinetochore structure and function.
  • This discovery advances our knowledge of the fundamental mechanisms governing chromosome segregation during mitosis.