Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comprehensive Benchmarking with Guidelines for Analyzing Transposable Element-derived RNA Expression.

Genomics, proteomics & bioinformatics·2026
Same author

Prediction of progressive local kyphosis following PVP/PKP for osteoporotic vertebral fractures based on the OF classification.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2026
Same author

Nano-enabled spatially selective protein degradation modulates lactate metabolism to potentiate antitumor immunity in liver cancer.

Nature nanotechnology·2026
Same author

Zynq-Based Hardware-Software Codesign Architecture for an Intelligent Hyperspectral Camera.

Sensors (Basel, Switzerland)·2026
Same author

Chirped-pulse coherent pulse stacking in a Yb-doped fiber laser for high-repetition-rate electron bunch shaping.

Optics express·2026
Same author

Hypervirulent <i>Klebsiella pneumoniae</i> induces liver abscess by promoting neutrophil extracellular trap formation through NLRP3 inflammasome activation.

Microbiology spectrum·2026

Related Experiment Video

Updated: Jun 2, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

MDC1 collaborates with TopBP1 in DNA replication checkpoint control.

Jiadong Wang1, Zihua Gong, Junjie Chen

  • 1Department of Experimental Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas 77030, USA.

The Journal of Cell Biology
|April 13, 2011
PubMed
Summary

This study reveals MDC1, a DNA double-strand break protein, interacts with TopBP1 to regulate DNA replication checkpoints and damage response pathways, crucial for maintaining genomic stability.

More Related Videos

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Related Experiment Videos

Last Updated: Jun 2, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Genetics

Background:

  • Topoisomerase Binding Protein 1 (TopBP1) is critical for regulating the DNA replication checkpoint.
  • The cellular response to DNA damage involves complex signaling cascades and protein interactions.

Purpose of the Study:

  • To identify novel TopBP1-interacting proteins.
  • To elucidate the role of MDC1 in the DNA replication checkpoint and DNA damage response.

Main Methods:

  • Co-immunoprecipitation assays to identify protein interactions.
  • Western blotting to detect protein accumulation.
  • Analysis of signaling pathways involved in DNA damage response.

Main Results:

  • MDC1, a key DNA double-strand break protein, was identified as a TopBP1-interacting protein.
  • The interaction between TopBP1 and MDC1 is mediated by the BRCT domain of TopBP1 and SDT repeats of MDC1.
  • TopBP1 accumulation at stalled replication forks is dependent on the H2AX/MDC1 signaling cascade.
  • MDC1 is essential for ATR-dependent Chk1 activation during replication stress.

Conclusions:

  • MDC1 plays a significant role in the DNA replication checkpoint.
  • MDC1 facilitates critical steps in both DNA damage response and replication checkpoint control.
  • The findings highlight a novel mechanism involving MDC1 and TopBP1 in maintaining genomic integrity.