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A new rapid method to measure human platelet cholesterol: a pilot study.

I Anita Jagroop1, Jahm Want Persaud, Dimitri P Mikhailidis

  • 1Department of Surgery, Division of Surgical and Interventional Science, Royal Free campus, University College London Medical School, University College London, London, UK.

Clinical and Applied Thrombosis/Hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis
|April 14, 2011
PubMed
Summary

A new, rapid method for measuring platelet cholesterol (PC) is reproducible and simpler than existing techniques. This advancement aids in assessing tissue cholesterol in vascular disease patients.

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Area of Science:

  • Biochemistry
  • Cardiovascular Research
  • Clinical Diagnostics

Background:

  • Platelet cholesterol (PC) is a potential biomarker for assessing tissue cholesterol in vascular disease.
  • Current methods for PC measurement are complex and time-consuming.
  • A simplified, rapid method for PC assessment is needed.

Purpose of the Study:

  • To develop and validate a rapid method for measuring platelet cholesterol (PC).
  • To assess the correlation of PC with various serum lipid parameters, including total cholesterol (TC), LDL-C, HDL-C, TG, and non-HDL-C.

Main Methods:

  • A novel, rapid method was developed to measure PC from platelet-rich plasma.
  • Repeatability and reproducibility of the PC assay were assessed.
  • Serum lipid profiles and PC levels were measured in 47 healthy participants.

Main Results:

  • The developed PC assay demonstrated good repeatability (CV 4.8%) and reproducibility (CV 7.5-8.1%).
  • Significant correlations were found between PC and serum LDL-C (r(s)=0.45), LDL-C/HDL-C ratio (r(s)=0.45), TG (r(s)=0.43), and non-HDL-C (r(s)=0.53).
  • PC levels in participants with elevated LDL-C (>2.6 mmol/L) were 377 ± 120 μmol/10(12) platelets.

Conclusions:

  • The new PC measurement technique is rapid, reproducible, and simpler than existing methods.
  • This method may facilitate serial sampling for monitoring PC changes in hypercholesterolemic patients.
  • Further extensive evaluation of this technique is warranted.