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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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Conserved interaction between transferrin and transferrin-binding proteins from porcine pathogens.

Leslie P Silva1, Ronghua Yu, Charles Calmettes

  • 1Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 1N4, Canada.

The Journal of Biological Chemistry
|April 14, 2011
PubMed
Summary

This study reveals how bacterial surface proteins bind to porcine transferrin (pTf) to acquire iron. The transferrin-binding protein B (TbpB) specifically targets the C lobe of pTf, crucial for iron uptake by Pasteurellaceae pathogens.

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Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Gram-negative Pasteurellaceae pathogens utilize surface receptor complexes to acquire iron from porcine transferrin (pTf).
  • The complex includes transferrin-binding protein A (TbpA) and transferrin-binding protein B (TbpB).
  • Mechanisms of pTf engagement and iron positioning for release remain unclear, especially across divergent strains.

Purpose of the Study:

  • To map the TbpB-pTf interface for multiple divergent strains of Actinobacillus plueropneumoniae and suis.
  • To characterize the binding interaction and validate the interface using site-directed mutagenesis.
  • To elucidate the role of TbpB in iron acquisition from pTf.

Main Methods:

  • Mass shift analysis to identify binding sites.
  • Molecular docking simulations to model the TbpB-pTf complex.
  • Site-directed mutagenesis to validate the interface and assess binding affinity.

Main Results:

  • TbpB binding to pTf was localized to the C lobe for divergent strains.
  • Binding involves sublobes C1 and C2, with C2 contributing more significantly to affinity.
  • Iron release from the TbpB-pTf complex is not spontaneous and requires other mediators.

Conclusions:

  • TbpB captures and presents iron-loaded pTf to the iron uptake pathway.
  • The findings provide insights into iron acquisition strategies of porcine pathogens.
  • This understanding can inform the development of novel antimicrobial strategies targeting iron uptake.