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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Updated: Jun 2, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Nucleic Acid programmable protein arrays: versatile tools for array-based functional protein studies.

Shane Miersch¹, Joshua LaBaer

¹Biodesign Institute at Arizona State University, Tempe, Arizona.

Current Protocols in Protein Science

|April 14, 2011

Summary

This summary is machine-generated.

The Nucleic Acid Programmable Protein Array (NAPPA) platform overcomes limitations of traditional protein microarrays. NAPPA enables in situ protein expression for diverse functional studies, offering a accessible and versatile research tool.

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Last Updated: Jun 2, 2026

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Published on: November 18, 2014

Area of Science:

Biochemistry
Molecular Biology
Genomics

Background:

Protein microarrays are valuable for studying gene product function.
Technical challenges with purified protein stability and dynamic range limit their use.
Existing methods hinder broad adoption of protein microarrays.

Purpose of the Study:

Introduce the Nucleic Acid Programmable Protein Array (NAPPA) as an alternative to traditional protein microarrays.
Detail the construction and application of the NAPPA platform.
Provide a reference for generating and utilizing NAPPA-based microarrays.

Main Methods:

Spotting protein-encoding plasmid DNA onto an array surface.
Utilizing cell-free expression systems for in situ protein generation.
Employing array printing and laser slide scanning for construction.

Main Results:

NAPPA circumvents limitations of spotted protein arrays.
The platform enables diverse functional protein studies (e.g., protein-small molecule interactions).
NAPPA is adaptable to various capture modalities and expression systems.

Conclusions:

NAPPA offers a viable alternative for functional protein studies.
The platform is accessible to labs with standard equipment.
NAPPA facilitates applications like screening for antibody biomarkers.