Related Experiment Video

Updated: Jun 2, 2026

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
05:45

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

Published on: January 9, 2020

Gene therapy for head and neck squamous cell carcinoma using KITENIN (KAI1 COOH-Terminal Interacting

Joon Kyoo Lee1, Dong-Hoon Lee, Eun Gene Sun

  • 1Department of Otolaryngology-Head and Neck Surgery, Chonnam National University Medical School, Hwasun Hospital, Hwasun-eup, Hwasun 519-763, Korea. joonkyoo@chonnam.ac.kr

Yonsei Medical Journal
|April 14, 2011
PubMed
Abstract

Insights

Gene therapy targeting KAI1 COOH-terminal interacting tetraspanin (KITENIN) effectively suppressed head and neck squamous cell carcinoma (SCC) progression. This approach reduced tumor cell invasion, migration, and proliferation in vitro.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Therapy

Background:

  • KAI1 COOH-terminal interacting tetraspanin (KITENIN) promotes metastasis in colon cancer and squamous cell carcinoma (SCC) models.
  • Previous studies showed KITENIN suppression via small interfering RNA (siRNA) reduced tumor progression in colon cancer.
  • Head and neck SCC represents a significant clinical challenge with unmet therapeutic needs.

Purpose of the Study:

  • To evaluate the efficacy of gene therapy targeting KITENIN in human head and neck SCC.
  • To investigate the impact of KITENIN suppression on SCC cell invasion, migration, and proliferation.

Main Methods:

  • Utilized the SNU-1041 human hypopharyngeal SCC cell line.
  • Measured KITENIN expression via Western blot analysis.
  • Administered siRNA targeting KITENIN (si-KITENIN) or scrambled control via transfection in vitro.
  • Assessed invasion, migration, and proliferation differences between groups.

Main Results:

  • KITENIN was highly expressed in SNU-1041 cells.
  • si-KITENIN treatment significantly reduced cell invasion (p<0.001).
  • Migration speed was significantly decreased in the si-KITENIN group (p<0.001).
  • Cell proliferation was significantly reduced by day three in the si-KITENIN group (p<0.001).
  • KITENIN protein expression was undetectable post-si-KITENIN transfection.

Conclusions:

  • Gene therapy targeting KITENIN demonstrates significant potential for treating head and neck SCC.
  • Suppression of KITENIN effectively inhibits key hallmarks of cancer progression in vitro.
  • An anti-KITENIN strategy warrants further investigation for head and neck SCC treatment.

More Related Videos

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity07:52

In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity

Published on: November 2, 2020

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

Related Concept Videos

Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Gene Therapy00:59

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be inserted. The...
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists01:28

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy.  SP binds and activates these...
Combination Therapies and Personalized Medicine02:50

Combination Therapies and Personalized Medicine

Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...