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Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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Related Experiment Video

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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
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NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

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Foxp3 interacts with c-Rel to mediate NF-κB repression.

Louiza Loizou1, Kristian G Andersen, Alexander G Betz

  • 1MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.

Plos One
|April 15, 2011
PubMed
Summary
This summary is machine-generated.

The transcription factor Foxp3 interacts with c-Rel, a key NF-κB component, influencing regulatory T cell function. Specific Foxp3 regions mediate binding to c-Rel and NFAT, impacting T cell development and suppression.

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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Foxp3 is a critical transcription factor for regulatory T cell (Treg) development and function.
  • Foxp3 interacts with various transcriptional regulators and epigenetic modifiers to orchestrate the Treg transcriptional program.
  • The precise molecular mechanisms of Foxp3 interactions, particularly with NF-κB family members, require further elucidation.

Purpose of the Study:

  • To investigate the direct interaction between Foxp3 and the NF-κB component c-Rel.
  • To identify the specific domains of Foxp3 involved in binding to c-Rel and NFAT.
  • To understand the functional implications of the Foxp3-c-Rel interaction in Treg biology.

Main Methods:

  • Co-immunoprecipitation assays to detect protein-protein interactions.
  • Deletion mutagenesis of Foxp3 to map interaction domains.
  • Analysis of Foxp3 and c-Rel binding affinities.

Main Results:

  • Foxp3 directly engages with the NF-κB component c-Rel, either alone or in a complex.
  • The N-terminal region of Foxp3 is essential for c-Rel binding, but not NFAT binding.
  • Deletion of the Foxp3 forkhead domain abolishes NFAT interaction but preserves c-Rel binding.

Conclusions:

  • Foxp3 forms a specific complex with c-Rel, mediated by its N-terminal region.
  • Distinct domains of Foxp3 govern interactions with c-Rel and NFAT, suggesting differential regulatory roles.
  • These findings provide insights into the dynamic regulation of Foxp3 activity by c-Rel during Treg development and homeostasis.