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Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
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Published on: December 8, 2017

Zebrafish embryo development in a microfluidic flow-through system.

Eric M Wielhouwer1, Shaukat Ali, Abdulrahman Al-Afandi

  • 1Institute of Biology, Leiden University, Sylvius Laboratory, Sylviusweg 72, 2333, BE, Leiden, The Netherlands.

Lab on a Chip
|April 15, 2011
PubMed
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Zebrafish embryos can now develop for 5 days in a novel microfluidic chip, offering a high-throughput, low-cost alternative to traditional methods. This lab-on-a-chip technology enables parallel experiments and high-quality imaging for biological research.

Area of Science:

  • Developmental Biology
  • Microfluidics
  • Animal Models

Background:

  • Zebrafish embryos are a cost-effective whole-animal model for research.
  • Current culture methods (microtitre plates) are invasive, reagent-intensive, and limit imaging.
  • Previous microfluidic attempts were static or prototypes, not flow-through systems for development.

Purpose of the Study:

  • To develop and prototype a lab-on-a-chip for continuous flow culture of zebrafish embryos.
  • To assess the feasibility of embryonic development in a microfluidic flow-through system.
  • To enable high-quality imaging and parallel experimentation with zebrafish embryos.

Main Methods:

  • Developed a specialized lab-on-a-chip from bonded borosilicate glass layers.
  • Cultured zebrafish embryos for 5 days with continuous buffer flow (0.005-0.04 MPa).

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Functional Cardiac Imaging in Zebrafish Embryos Using Standard Microscopy and Video Analysis: Applications in Environmental and Biomedical Research
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  • Performed acute ethanol exposure tests to validate assay replication.

    • Main Results:

    • Zebrafish embryos successfully developed for 5 days in the microfluidic chip.
    • 100% survival rates achieved with optimized buffer flow (2 µL/well/min).
    • High-quality imaging was possible, and ethanol tests replicated microtitre plate results.

    Conclusions:

    • The developed microfluidic chip supports zebrafish embryonic development, offering a viable alternative to static culture.
    • This technology enables massive, parallel experiments with zebrafish larvae at high speed and low cost.
    • The system facilitates high-quality imaging and assay replication in a compact format (< credit card size).