Related Experiment Video
Updated: Jun 2, 2026
Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
Published on: January 22, 2020
Application of quartz crystal microbalance to study the impact of pH and ionic strength on protein-silicone oil
Nitin Dixit1, Kevin M Maloney, Devendra S Kalonia
1Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, 69N Eagleville Road, Unit 3092, Storrs, CT 06269, USA.
Abstract:
In this study, we have used quartz crystal microbalance (QCM) to quantitate the adsorption of a protein on silicone oil coated surfaces as a function of protein concentration, pH and ionic strength using a 5 MHz quartz crystal. Protein adsorption isotherms were generated at different solution pH and ionic strengths. Surface saturation concentrations were selected from adsorption isotherms and used to generate adsorption profiles from pH 3.0 to 9.0, and at ionic strengths of 10 mM and 150 mM. At low ionic strength (10mM) and pH 5.0 (close to the isoelectric point of the protein), maximum adsorption of protein to the silicone oil surface was observed. At higher ionic strength (150 mM), no significant pH influence on adsorption was observed. QCM could be used as a reliable technique to study the binding of proteins to silicone oil coated surfaces.
More Related Videos
07:11Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
Published on: November 1, 2018
12:38Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
Published on: April 11, 2021