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Updated: Jun 2, 2026

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Antimicrobial effect of chitosan nanoparticles on streptococcus mutans biofilms
Luis E Chávez de Paz1, Anton Resin, Kenneth A Howard
1Department of Oral Biology, Faculty of Odontology, Malmö University, SE-20506 Malmö, Sweden. luis.chavez.de.paz@mah.se
Abstract:
Nanoparticle complexes were prepared from chitosans of various molecular weights (MW) and degrees of deacetylation (DD). The antimicrobial effect was assessed by the Live/Dead BacLight technique in conjunction with confocal scanning laser microscopy (CSLM) and image analysis. Nanocomplexes prepared from chitosans with high MW showed a low antimicrobial effect (20 to 25% of cells damaged), whereas those prepared from low-MW chitosans showed high antimicrobial effect (>95% of cells damaged).
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