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Membrane alterations following interferon treatment.

E H Chang, E F Grollman, F T Jay

    Advances in Experimental Medicine and Biology
    |January 1, 1978
    PubMed
    Summary
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    Interferon treatment alters cell membranes, impacting ganglioside exposure and particle count. This antiviral response involves changes in plasma membrane density and viral glycoprotein composition.

    Area of Science:

    • Cell Biology
    • Virology
    • Immunology

    Background:

    • Interferons are cytokines with antiviral properties.
    • Cellular responses to interferons are complex and involve membrane alterations.
    • Understanding these changes is crucial for antiviral therapies.

    Purpose of the Study:

    • To investigate the effects of interferon treatment on uninfected cell plasma membranes.
    • To analyze changes in ganglioside exposure and membrane particle dynamics.
    • To characterize viral particles produced by interferon-treated cells.

    Main Methods:

    • Galactose oxidase treatment and tritium incorporation to assess surface gangliosides.
    • Electron microscopy to quantify intramembranous particles.
    • Buoyant density centrifugation to analyze plasma membrane properties.

    Related Experiment Videos

  • Analysis of viral glycoproteins from produced Moloney murine leukemia virus.

    • Main Results:

    • Interferon treatment altered ganglioside exposure on Ly and KB cell membranes.
    • AKR-2B mouse cells showed increased intramembranous particles, kinetics matching antiviral activity.
    • Plasma membrane buoyant density increased significantly in treated cells.
    • Moloney murine leukemia virus from treated cells had altered glycoprotein composition, including a prominent 85,000 MW glycoprotein.

    Conclusions:

    • Interferon induces significant changes in the plasma membrane of uninfected cells.
    • These membrane alterations are linked to the establishment of antiviral activity.
    • Changes in viral glycoproteins suggest interferon influences viral assembly or maturation.