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Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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Electron Microscope Tomography and Single-particle Reconstruction

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Electron Tomography
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Introduction: MRI and CT scans are crucial advancements in medical imaging techniques, playing a vital role in diagnosing conditions related to the gastrointestinal (GI) system. Each scan serves distinct purposes, targets specific areas, and requires unique nursing duties.
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Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...

Positron Emission Tomography

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Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
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High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

Cellular tomography.

Andreas Hoenger¹, Cédric Bouchet-Marquis

¹The Boulder Laboratory for 3-D Electron Microscopy of Cells, Molecular, Cellular & Developmental Biology, University of Colorado at Boulder, Boulder, Colorado, USA.

Advances in Protein Chemistry and Structural Biology

|April 20, 2011

Summary

This summary is machine-generated.

Cellular tomography advances enable detailed 3D imaging of cells and organelles. New methods allow direct imaging of vitrified specimens, improving structural analysis in electron microscopy.

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Area of Science:

Cell Biology
Microscopy Techniques
Structural Biology

Background:

Traditional electron microscopy (EM) involves chemical fixation or freezing, followed by embedding and sectioning.
Recent advancements include tomographic 3D reconstruction and improved preparation of vitrified specimens for cryo-EM.
Modern computing power facilitates handling large datasets for tomographic analysis.

Purpose of the Study:

To provide an overview of cellular tomography techniques.
To discuss the evolution and applications of electron tomography in cell biology.
To categorize current cellular tomography approaches.

Main Methods:

Tomographic 3D reconstruction of cellular structures.
Preparation of plastic-embedded sections (chemically fixed or freeze-substituted).
Cryo-electron microscopy (cryo-EM) for imaging vitrified specimens (sectioned or intact).

Main Results:

Cellular tomography is classified into four main approaches based on specimen preparation and imaging conditions.
Tomography is well-suited for large, irregular cellular structures like organelles and macromolecular assemblies.
Cryo-EM and cryo-tomography enable imaging of intact vitrified cells and thin sections under cryo-conditions.

Conclusions:

Cellular tomography offers powerful tools for high-resolution 3D structural analysis of cells.
Advancements in specimen preparation and imaging technology have expanded the scope of cellular tomography.
The field benefits significantly from improved computational methods for data processing.