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Related Concept Videos

Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
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Related Experiment Video

Updated: Jun 2, 2026

Epon Post Embedding Correlative Light and Electron Microscopy
08:47

Epon Post Embedding Correlative Light and Electron Microscopy

Published on: January 12, 2024

Correlative light-electron microscopy.

Dorit Hanein1, Niels Volkmann

  • 1Bioinformatics and Systems Biology Program, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.

Advances in Protein Chemistry and Structural Biology
|April 20, 2011
PubMed
Summary
This summary is machine-generated.

New microscopy techniques correlate dynamic biological processes with their structures in real-time. This allows for a deeper understanding of cellular functions and pathways at high resolution.

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Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

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Related Experiment Videos

Last Updated: Jun 2, 2026

Epon Post Embedding Correlative Light and Electron Microscopy
08:47

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Published on: January 12, 2024

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
11:16

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles

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Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets
09:10

Correlative Light and Electron Microscopy (CLEM) as a Tool to Visualize Microinjected Molecules and their Eukaryotic Sub-cellular Targets

Published on: May 4, 2012

Area of Science:

  • Correlative light and electron microscopy (CLEM)
  • In situ structural biology
  • Dynamic biological processes

Background:

  • Understanding cellular mechanisms requires correlating dynamic events with static structures.
  • Traditional imaging methods often lack the resolution or temporal information needed for such correlations.
  • Advances in microscopy are enabling simultaneous acquisition of dynamic and structural data.

Purpose of the Study:

  • To highlight recent advancements in correlative imaging techniques.
  • To demonstrate the quantitative correlation of dynamic biological pathways with high-resolution structures.
  • To showcase the ability to observe these phenomena within the same spatiotemporal window.

Main Methods:

  • Integration of light microscopy (LM) for dynamic observation.
  • Application of electron microscopy (EM) for high-resolution structural details.
  • Development of techniques for in situ correlation of LM and EM data.

Main Results:

  • Successful correlation of dynamic biological pathway states with their structural underpinnings.
  • Quantitative analysis of biological processes at high resolution in their native environment.
  • Demonstration of simultaneous imaging of dynamic and structural information.

Conclusions:

  • Correlative LM-EM techniques offer unprecedented insights into biological systems.
  • These methods facilitate a deeper understanding of cellular functions by linking dynamics to structure.
  • Future research can leverage these advances for detailed mechanistic studies of biological pathways.