[Determination of the phospholipase C gene in Pseudomonas aeruginosa using DNA hybridization]

The authors tested the possibility to use the method of colony hybridization for screening of thermolabile haemolysin (phospholipase C) in strains of Pseudomonas aeruginosa. As a radioactively labelled probe they used SmaI fragments of the pGV26 plasmid, which contains the 6.3 kb fragment of the chromosome of strains P. aeruginosa PAD1 with the gene for phospholipase C.