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Carboxyl-terminal deletion analysis of the Streptococcus mutans glucosyltransferase-I enzyme
1Department of Pediatric Dentistry, University of Texas Health Science Center, San Antonio 78284.
FEMS Microbiology Letters
|November 1, 1990
Abstract:
Sequential deletion of the carboxyl-terminal amino acids (including the six direct repeating units) of the glucosyltransferase-I (GTF-I) enzyme of Streptococcus mutans revealed differential effects on sucrase and GTF activities. Removal of all but one repeating unit resulted in a truncated enzyme with significant sucrase activity but no detectable GTF activity. These results are compatible with the presence of two functional domains in the enzyme.