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Related Concept Videos

Inhibitors of Viral Protein Synthesis01:30

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...
Inhibitors Of Virion Release01:25

Inhibitors Of Virion Release

Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...
Inhibitors of Virion Maturation and Assembly01:19

Inhibitors of Virion Maturation and Assembly

As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
Coronavirus01:29

Coronavirus

Coronaviruses, including the severe acute respiratory syndrome coronavirus (SARS-CoV), are enveloped viruses characterized by their single-stranded, positive-sense RNA genome and helical nucleocapsid structure. The hallmark of these viruses is their club-shaped spike (S) glycoproteins that protrude from the viral envelope, facilitating attachment to host cells. Typically, coronaviruses infect the upper respiratory tract, often causing mild or asymptomatic disease. However, certain strains like...

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Related Experiment Video

Updated: Jun 2, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Blocking eIF4E-eIF4G interaction as a strategy to impair coronavirus replication.

Regina Cencic1, Marc Desforges, David R Hall

  • 1McIntyre Medical Sciences Building, Rm. 810, 3655 Promenade Sir William Osler, McGill University, Montreal, Quebec, Canada H3G 1Y6.

Journal of Virology
|April 22, 2011
PubMed
Summary
This summary is machine-generated.

Researchers identified a molecule that inhibits eukaryotic initiation factor 4F (eIF4F) activity, significantly reducing human coronavirus 229E (HCoV-229E) replication by blocking cap-dependent translation. This discovery supports targeting eIF4F to combat coronavirus infections.

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Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

Related Experiment Videos

Last Updated: Jun 2, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
05:23

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization

Published on: December 23, 2020

Area of Science:

  • Virology
  • Molecular Biology
  • Drug Discovery

Background:

  • Coronaviruses are RNA viruses causing various diseases, including common colds.
  • Coronavirus replication relies on subgenomic mRNAs (sgmRNAs) and cap-dependent translation.
  • Eukaryotic initiation factor 4F (eIF4F) is crucial for recruiting ribosomes during translation.

Purpose of the Study:

  • To discover compounds inhibiting eIF4F activity.
  • To identify a molecule that blocks the interaction between eIF4E and eIF4G.
  • To assess the inhibitor's effect on human coronavirus 229E (HCoV-229E) replication.

Main Methods:

  • Ultrahigh-throughput screening for eIF4F inhibitors.
  • Characterization of a molecule inhibiting eIF4E-eIF4G interaction.
  • Assay of HCoV-229E replication in the presence of the inhibitor.

Main Results:

  • A novel molecule was identified that inhibits eIF4F by preventing eIF4E and eIF4G interaction.
  • This inhibitor blocked cap-dependent translation.
  • Significant reduction in HCoV-229E replication, including decreased infected cells and virus titers.

Conclusions:

  • Targeting the eIF4F complex is a viable strategy to inhibit coronavirus infection.
  • The identified molecule effectively reduces HCoV-229E replication.
  • Further research into eIF4F inhibitors holds promise for antiviral therapies.