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A Customizable Antibody Delivery Strategy Using Fc-Affinity Ligands.

Daniela Isaacs-Bernal1,2, Noor E Bahsoun1,2, Lia Huo2,3

  • 1Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario M5S 3E5, Canada.

ACS Biomaterials Science & Engineering
|March 6, 2026
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Summary
This summary is machine-generated.

This study demonstrates a new method for controlled protein delivery using hydrogels and specific peptide ligands to release antibodies. This affinity-controlled release strategy offers tunable delivery of therapeutic antibodies.

Keywords:
affinity ligandantibodybevacizumabcontrolled releasehydrogelsprotein delivery

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Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Protein Engineering

Background:

  • Controlled release of therapeutic proteins, particularly antibodies, from hydrogel systems is crucial for effective treatment.
  • Harnessing noncovalent interactions offers a versatile platform for affinity-controlled protein delivery.

Purpose of the Study:

  • To develop and evaluate a novel strategy for the controlled release of native antibodies from hydrogels using affinity interactions.
  • To engineer peptide ligands targeting the Fc region of human IgG1 for immobilization within hydrogels.

Main Methods:

  • Engineered two Fc-binding ligands (FcL1 and FcLPEG) with varying affinities (KD) for the Fc region of human IgG1.
  • Immobilized ligands within chemically cross-linked hyaluronan-oxime hydrogels and physically cross-linked hyaluronan-methylcellulose hydrogels.
  • Investigated the release kinetics of therapeutic antibodies (bevacizumab, adalimumab) from the hydrogel systems.

Main Results:

  • Controlled release of bioactive bevacizumab was achieved using the higher-affinity FcL1 ligand in a hyaluronan-oxime hydrogel.
  • The lower-affinity FcLPEG ligand did not facilitate controlled release.
  • Tunable release of multiple IgG1 antibodies over a 7-day period was demonstrated in a hyaluronan-methylcellulose hydrogel system.

Conclusions:

  • The developed affinity-controlled release strategy is broadly applicable for tuning antibody delivery from hydrogels.
  • This approach enables precise control over the release of therapeutic antibodies, enhancing their potential clinical utility.