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Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
Positive Regulator Molecules01:45

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

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Related Experiment Video

Updated: Jun 2, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

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Published on: May 3, 2018

CDK6 kinase activity is required for thymocyte development.

Miaofen G Hu1, Amit Deshpande, Nicolette Schlichting

  • 1Molecular Oncology Research Institute, Tufts Medical Center, Boston, MA, USA.

Blood
|April 22, 2011
PubMed
Summary
This summary is machine-generated.

Cyclin-dependent kinase-6 (CDK6) kinase activity is crucial for thymocyte development, partly by influencing Notch signaling. Targeting CDK6 offers a potential therapeutic strategy for lymphoid malignancies.

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Published on: June 15, 2017

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Cyclin-dependent kinase-6 (CDK6) plays a role in early thymocyte development and tumorigenesis.
  • Understanding CDK6's precise function in thymocyte development requires mechanistic investigation.

Purpose of the Study:

  • To mechanistically dissect the role of CDK6 kinase activity in thymocyte development using mutant knock-in mice.
  • To investigate the interplay between CDK6 and Notch signaling pathways in hematopoietic stem cells and thymocytes.

Main Methods:

  • Generation and analysis of mice expressing kinase-dead (Cdk6(K43M)) and hyperactive (Cdk6(R31C)) Cdk6 alleles.
  • Flow cytometry analysis of thymocytes and hematopoietic stem and progenitor cells (LSK).
  • Assessment of Notch signaling pathway components and target gene expression, including CD25.

Main Results:

  • Kinase-dead Cdk6 (Cdk6(K43M)) mice showed reduced thymocytes and LSK cells.
  • Hyperactive Cdk6 (Cdk6(R31C)) mice exhibited increased proliferation but also elevated apoptosis.
  • Inactive CDK6 disrupted Notch-dependent survival, proliferation, and differentiation, leading to CD25 up-regulation; CD25 knockout rescued defects in Cdk6(K43M) mice.

Conclusions:

  • CDK6 kinase activity is essential for normal thymocyte development, partly by modulating Notch target gene expression.
  • CDK6 acts as a downstream mediator of Notch signaling in this context.
  • CDK6 kinase activity represents a potential therapeutic target for human lymphoid malignancies.