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Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
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Related Experiment Video

Updated: Jun 2, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Spectral domain fluorescence coherence phase microscopy.

Andra St Quintin1, Lukas-Karim Merhi, Marinko V Sarunic

  • 1School of Engineering Science, Simon Fraser University, 8888 University Drive, Burnaby, Canada, V5A 1S6.

Applied Optics
|April 22, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces spectral domain fluorescence coherence phase microscopy for nanoscale motion detection. It combines phase analysis with fluorescence signals, offering molecular specificity for applications like cell rheology.

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Last Updated: Jun 2, 2026

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Area of Science:

  • Biophysics
  • Optical Microscopy
  • Nanotechnology

Background:

  • Spectral domain phase microscopy (SDPM) extends low-coherence interferometry for nanoscale displacement measurements.
  • Existing SDPM signals lack molecular specificity, relying on structural images.
  • There is a need for functional imaging with molecular specificity in microscopy.

Purpose of the Study:

  • To expand phase analysis to fluorescence self-interference signals.
  • To achieve molecular specificity in nanoscale motion detection.
  • To demonstrate a new microscopy technique for functional imaging.

Main Methods:

  • Developed spectral domain fluorescence coherence phase microscopy (SD-FCPM).
  • Applied phase processing to fluorescence self-interference signals.
  • Utilized fluorescent particles for motion detection experiments.

Main Results:

  • Achieved nanoscale resolution motion detection of fluorescent particles.
  • Demonstrated a signal-to-noise ratio limited resolution of approximately 10 nm.
  • Successfully combined phase processing with fluorescence self-interference.

Conclusions:

  • Spectral domain fluorescence coherence phase microscopy is feasible.
  • The technique provides functional information with molecular specificity.
  • Potential applications include cell rheology and other biological studies.