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Related Experiment Video

Updated: Jun 2, 2026

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
09:12

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Published on: March 10, 2020

The versatile Legionella effector protein DrrA.

Roger S Goody1, Matthias P Müller, Stefan Schoebel

  • 1Max-Planck-Institute of Molecular Physiology; Department of Physical Biochemistry; Dortmund, NRW Germany.

Communicative & Integrative Biology
|April 22, 2011
PubMed
Summary
This summary is machine-generated.

The Legionella bacterium uses the DrrA protein to modify the host cell

Keywords:
GEFLegionellaLegionella containing vacuoleRabadenylylationphosphoinositidevesicular trafficking

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Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Published on: February 22, 2017

Area of Science:

  • Microbiology
  • Cell Biology
  • Molecular Biology

Background:

  • Legionella pneumophila is a human pathogen that infects host cells.
  • The bacterium injects effector proteins, like DrrA, into the host cell.
  • DrrA manipulates host cell processes, including vesicular trafficking.

Purpose of the Study:

  • To summarize the properties of the Legionella effector protein DrrA.
  • To investigate the mechanism by which DrrA modifies the host protein Rab1.
  • To speculate on the consequences of Rab1 modification for host-pathogen interactions.

Main Methods:

  • The study is a review and speculation based on existing literature.
  • Focuses on the known functions and biochemical properties of DrrA.
  • Discusses the implications of DrrA's interaction with Rab1.

Main Results:

  • DrrA binds to the Legionella-containing vacuole (LCV).
  • DrrA recruits and activates the host protein Rab1, a regulator of vesicular trafficking.
  • DrrA adenylylates Rab1 at Tyr77, prolonging its active state and interfering with its deactivation.

Conclusions:

  • DrrA's adenylylation of Rab1 likely disrupts normal host vesicular trafficking.
  • This disruption facilitates Legionella survival and replication within the host cell.
  • Understanding DrrA's mechanism provides insights into bacterial pathogenesis and host cell manipulation.