Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutation in Bruton Tyrosine Kinase (BTK) A428D confers resistance To BTK-degrader therapy in chronic lymphocytic leukemia.

Leukemia·2024
Same author

Calibrator material selection: a key criteria during biomarker assay method development.

Bioanalysis·2021
Same author

Novel techniques and the new age of biomarkers: panel discussion report.

Bioanalysis·2021
Same author

2020 White Paper on Recent Issues in Bioanalysis: BMV of Hybrid Assays, Acoustic MS, HRMS, Data Integrity, Endogenous Compounds, Microsampling and Microbiome (<u>Part 1</u> - Recommendations on Industry/Regulators Consensus on BMV of Biotherapeutics by LCMS, Advanced Application in Hybrid Assays, Regulatory Challenges in Mass Spec, Innovation in Small Molecules, Peptides and Oligos).

Bioanalysis·2021
Same author

Immunoaffinity microflow liquid chromatography/tandem mass spectrometry for the quantitation of PD1 and PD-L1 in human tumor tissues.

Rapid communications in mass spectrometry : RCM·2020
Same author

2019 White Paper on Recent Issues in Bioanalysis: Chromatographic Assays (Part 1 - Innovation in Small Molecules and Oligonucleotides & Mass Spectrometric Method Development Strategies for Large Molecule Bioanalysis).

Bioanalysis·2019

Related Experiment Video

Updated: Feb 19, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K

Optimization of Exactive Orbitrap™ acquisition parameters for quantitative bioanalysis.

Richard L Wong1, Baomin Xin, Timothy Olah

  • 1Bioanalytical Research, Research & Development, Bristol-Myers Squibb, 311 Pennington-Rocky Hill Road, Pennington, NJ 08534, USA. richard.wong@bms.com

Bioanalysis
|April 23, 2011
PubMed
Summary

Optimizing Orbitrap™ mass spectrometry for quantitation in drug discovery shows promising results. This technique achieves low nanomolar limits of quantitation, suitable for bioanalytical applications.

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.8K

Related Experiment Videos

Last Updated: Feb 19, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.3K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.8K

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Proteomics

Background:

  • Orbitrap™ mass spectrometry is a powerful tool for qualitative analysis.
  • Its quantitative capabilities are less understood compared to established techniques.
  • Further research is needed to fully leverage its potential in quantitative applications.

Purpose of the Study:

  • To investigate optimal acquisition parameters for quantitative analysis using Orbitrap™ mass spectrometry.
  • To evaluate the performance of Orbitrap™ for bioanalytical applications in drug discovery.

Main Methods:

  • Acquisition parameters were optimized for propranolol, reserpine, leucine enkephalin, and neurotensin.
  • Analysis was performed on mouse plasma samples.
  • Quantitative performance was assessed, including limits of quantitation and dynamic range.

Main Results:

  • Achieved lower limits of quantitation in the 1-3 nM range.
  • Demonstrated a quantitative linear dynamic range spanning four orders of magnitude.
  • Performance is adequate for most bioanalytical drug discovery applications.

Conclusions:

  • Increasing ion population enhances detection and lowers the limit of quantitation.
  • Higher ion populations may negatively impact the upper limit of quantitation.
  • A deeper understanding of operating parameters is crucial for optimizing experimental design and quantitative performance.