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Updated: Jun 2, 2026

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
Published on: March 18, 2017
Physical properties of mesenchymal stem cells are coordinated by the perinuclear actin cap
Takanori Kihara1, Seyed Mohammad Ali Haghparast, Yuji Shimizu
1Department of Mechanical Science and Bioengineering, Graduate School of Engineering Science, Osaka University, Machikaneyama, Toyonaka, Japan. takanori.kihara@gmail.com
Abstract:
Mesenchymal stem cells (MSCs) have been extensively investigated for their applications in regenerative medicine. Successful use of MSCs in cell-based therapies will rely on the ability to effectively identify their properties and functions with a relatively non-destructive methodology. In this study, we measured the surface stiffness and thickness of rat MSCs with atomic force microscopy and clarified their relation at a single-cell level. The role of the perinuclear actin cap in regulating the thickness, stiffness, and proliferative activity of these cells was also determined by using several actin cytoskeleton-modifying reagents. This study has helped elucidate a possible link between the physical properties and the physiological function of the MSCs, and the corresponding regulatory role of the actin cytoskeleton.
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