Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human lymphocytes expressing a TCR gamma/delta.

E Ciccone1, C E Grossi, W Malorni

  • 1Istituto Nazionale per la Ricerca sul Cancro, Genoa, Italy.

Annali Dell'Istituto Superiore Di Sanita
|January 1, 1990
PubMed
Summary

Gamma delta T cells, a unique lymphocyte subset, exhibit potent anti-tumor activity and recognize specific antigens. Two distinct subsets, identified by monoclonal antibodies, show varied distribution in blood and tissues.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent Advances in Human Natural Killer Cells.

International archives of allergy and immunology·2021
Same author

Insect antimicrobial peptides: potential weapons to counteract the antibiotic resistance.

Cellular and molecular life sciences : CMLS·2021
Same author

Tracheocutaneous fistula in patients undergoing supracricoid partial laryngectomy: the role of chronic aspiration.

Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale·2015
Same author

Functional and genetic aberrations of in vitro-cultured marrow-derived mesenchymal stromal cells of patients with classical Philadelphia-negative myeloproliferative neoplasms.

Leukemia·2014
Same author

KIR and KIR ligand polymorphism: a new area for clinical applications?

Tissue antigens·2014
Same author

Cell surface expression of activating receptors and co-receptors on peripheral blood NK cells in systemic autoimmune diseases.

Scandinavian journal of rheumatology·2012

Area of Science:

  • Immunology
  • Cell Biology
  • T lymphocyte subsets

Background:

  • A minor subset of T lymphocytes expresses a T-cell receptor (TCR) composed of gamma and delta chains.
  • These TCR gamma/delta + cells possess distinct phenotypic and functional characteristics compared to conventional T cells, notably lacking both CD4 and CD8 antigens.

Purpose of the Study:

  • To characterize the functional capabilities and antigenic recognition of TCR gamma/delta + T cells.
  • To identify and differentiate subsets within the TCR gamma/delta + T cell population using specific monoclonal antibodies.

Main Methods:

  • Cloning of CD4-CD8- peripheral blood lymphocytes under limiting dilution conditions.
  • Functional assays including proliferation in mixed lymphocyte culture (MLC) and cytolytic activity against tumor target cells.

Related Experiment Videos

  • Use of monoclonal antibodies (e.g., BB3, delta-TCS-1) to identify and activate distinct TCR gamma/delta + cell subsets, assessing responses via intracellular Ca2+ flux and lymphokine production.

    • Main Results:

    • CD4-CD8- peripheral blood lymphocytes were confirmed to be homogeneously composed of cytolytic cells effective against tumor targets.
    • TCR gamma/delta + cells demonstrated antigen recognition, evidenced by proliferation in MLC and specific lysis of PHA-induced blast cells bearing alloantigens.
    • Two distinct TCR gamma/delta + cell subsets were identified: BB3-reactive and delta-TCS-1-reactive. The BB3-reactive subset is prevalent in peripheral blood, while delta-TCS-1-reactive cells are more common in tissues.

    Conclusions:

    • TCR gamma/delta + T cells are a distinct population of cytotoxic lymphocytes capable of specific antigen recognition.
    • The identification of distinct TCR gamma/delta + subsets with differential tissue distribution provides insights into their specialized roles in immunity.