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Related Concept Videos

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung15:01

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

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We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition...
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Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software11:30

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

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We present a label-free live imaging protocol using transmitted light microscopy techniques to capture images, analyze and quantify growth kinetics of the filamentous fungus A. nidulans in both submerged cultures and solid media. This protocol can be used in conjunction with fluorescence...
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Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions09:42

Infection of Zebrafish Larvae with Aspergillus Spores for Analysis of Host-Pathogen Interactions

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This protocol describes an Aspergillus infection model in zebrafish larvae. Aspergillus spores are microinjected into the hindbrain of larvae, and chemical treatment is used to induce immunosuppression. Infection progression is monitored via a daily imaging setup to monitor fungal growth and immune responses as well as enumeration of live spores by colony forming unit...
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Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry09:43

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

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This protocol provides a fast and reliable method to quantitatively measure phagocytosis of Aspergillus fumigatus conidia by human primary phagocytes using flow cytometry and to discriminate phagocytosis of conidia from mere adhesion to...
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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

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Here we present a protocol to analyze Aspergillus flavus growth and aflatoxin production in maize kernels expressing an antifungal protein.  Using a GFP-expressing A. flavus strain we monitored the infection and spread of the fungus in mature kernels in real time. The assay is rapid, reliable, and...
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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

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We demonstrate a method for the analysis of aflatoxins and transgene expression in peanut seeds that contain RNA-interference signals for silencing aflatoxin-synthesis genes in the fungus Aspergillus flavus. RNAi-mediated control of mycotoxins in plants has not been reported...
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Related Experiment Video

Updated: Jan 19, 2026

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
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Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

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Aspergillus flavus.

Saori Amaike1, Nancy P Keller

  • 1Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706, USA.

Annual Review of Phytopathology
|April 26, 2011
PubMed
Summary

Aspergillus flavus poses a significant threat to agriculture and human health due to aflatoxin contamination and opportunistic infections. Research is advancing our understanding of its pathogenicity and control strategies.

Area of Science:

  • Mycology
  • Plant Pathology
  • Medical Mycology

Background:

  • Aspergillus flavus is a soil fungus contaminating crops with aflatoxin, a carcinogen.
  • It is an opportunistic pathogen causing aspergillosis, particularly in immunocompromised individuals.
  • Genomic data reveals numerous secondary metabolite clusters regulated by key factors like LaeA and VeA.

Purpose of the Study:

  • To review the agricultural and medical threats posed by Aspergillus flavus.
  • To summarize recent advances in understanding A. flavus pathogenicity and control.

Main Methods:

  • Genomic analysis of A. flavus.
  • Elucidation of pathogenicity factors including secondary metabolites and enzymes.
  • Investigation of host-pathogen interactions and quorum-like development.

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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

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Related Experiment Videos

Last Updated: Jan 19, 2026

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry
09:43

Measuring Phagocytosis of Aspergillus fumigatus Conidia by Human Leukocytes using Flow Cytometry

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Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the &#945;-amylase Inhibitor from Lablab purpureus L.
09:21

Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.

Published on: February 15, 2019

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RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem
09:44

RNAi-mediated Control of Aflatoxins in Peanut: Method to Analyze Mycotoxin Production and Transgene Expression in the Peanut/Aspergillus Pathosystem

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  • Evaluation of atoxic strains for competitive exclusion in crop protection.

    • Main Results:

    • A. flavus possesses 55 secondary metabolite clusters.
    • Pathogenicity is linked to secondary metabolite production, enzyme activity, and host crosstalk.
    • Atoxic strains show promise for biocontrol via competitive exclusion.

    Conclusions:

    • Aspergillus flavus presents a dual threat to agriculture and public health.
    • Advances in genomics and pathogenicity research are crucial for developing effective control measures.
    • Competitive exclusion using atoxic strains offers a promising strategy for managing A. flavus in agriculture.