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Related Experiment Video

Updated: Jun 2, 2026

Inducing Acute Liver Injury in Rats via Carbon Tetrachloride (CCl4) Exposure Through an Orogastric Tube

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Fatal intoxication due to brucine.

Jörg Teske¹, Jens-Peter Weller, Urs-Vito Albrecht

¹Institute of Legal Medicine, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. teske.joerg@mh-hannover.de

Journal of Analytical Toxicology

|April 26, 2011

Summary

This study presents a sensitive liquid chromatography-tandem mass spectrometry method for identifying brucine. The method accurately quantified brucine in various biological samples from a lethal intoxication case.

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Area of Science:

Analytical Chemistry
Forensic Toxicology

Background:

Brucine is a toxic alkaloid found in Strychnos species.
Accurate quantification of brucine is crucial for clinical and forensic toxicology.

Observation:

A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed.
Solid-phase extraction was employed for sample preparation from human serum.

Findings:

The method demonstrated excellent sensitivity with limits of detection (LOD) and quantification (LOQ) of 0.12 ng/mL and 0.23 ng/mL, respectively.
Brucine was quantified in multiple biological matrices (blood, urine, bile, liver, cerebellum, cerebrum, stomach contents) in a fatal case.

Implications:

This validated LC-MS/MS method provides a reliable tool for brucine analysis in forensic and clinical settings.
The findings highlight the utility of the method in post-mortem investigations involving brucine poisoning.