Related Experiment Video
Updated: Jun 2, 2026
Network Pharmacology Prediction and Metabolomics Validation of the Mechanism of Fructus Phyllanthi against Hyperlipidemia
Published on: April 7, 2023
[Determination of the content of phloridzin using reversed-phase high-performance liquid chromatography]
Jian-Rong He1, Xue-Ying Liu, Qing-Wei Wang
1Department of Pharmacy, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
Objective:
To establish a reversed-phase high-performance liquid chromatographic (RP-HPLC) method for determination of phloridzin content.
Methods:
A RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3 (4.6×150 mm, 5 µm) column with the detection wavelength of 288 nm, flow rate of 1.0 ml/min, and column temperature of 25 degrees celsius;.
Results:
The result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 µg/ml. The regression equation was Y=46.370 X-0.6728 (r=0.9999, n=3). The average recovery of phloridzin was 99.40% with the relative standard deviations (RSD) of 0.67%.
Conclusion:
This method is simple, quick and accurate for determination of phloridzin content.
Related Concept Videos
01:11High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
01:12Chromatographic Methods: Classification
Chromatographic techniques are typically named by...