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Published on: February 28, 2017
Src is sufficient, but not necessary, for osteopontin induction in osteoblasts
Jessica M Morgan1, Alice Wong, Damian C Genetos
1Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Biorheology
|April 26, 2011
Summary
Bone cells use mechanical signaling for adaptation. While Src kinase is activated by mechanical load and influences osteopontin expression statically, it is not essential for load-induced osteopontin expression in bone cells.
Area of Science:
- Biochemistry
- Cell Biology
- Biomechanical Engineering
Background:
- Bone adaptation to mechanical load is crucial for skeletal health.
- Mechanotransduction in bone cells involves signaling pathways at focal adhesions.
- The role of Src tyrosine kinase in osteoblast mechanotransduction remains unclear.
Purpose of the Study:
- To investigate the effect of oscillatory fluid flow on Src phosphorylation in osteoblasts.
- To determine Src's role in the osteopontin response to fluid flow.
- To examine the impact of constitutively active Src on osteopontin expression.
Main Methods:
- Osteoblasts were exposed to oscillatory fluid flow.
- Src phosphorylation at tyrosine 416 was measured.
- Osteopontin mRNA levels were assessed after Src manipulation.
- ERK1/2 phosphorylation was analyzed.
Main Results:
- Oscillatory fluid flow significantly increased Src phosphorylation at Y416 after 15 minutes.
- Constitutively active Src increased Src-Y416 phosphorylation and osteopontin mRNA under static conditions.
- Src inhibition did not affect flow-induced osteopontin expression or ERK1/2 phosphorylation.
Conclusions:
- Src activity is induced by shear stress and regulates osteopontin expression statically.
- Src is not required for the osteopontin response to mechanical load in osteoblasts.
