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Related Concept Videos

Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
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Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
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Cell culture contamination.

Glyn N Stacey1

  • 1National Institute for Biological Standards and Control, South Mimms, Herts, UK. gstacey@nibsc.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|April 26, 2011
PubMed
Summary
This summary is machine-generated.

Preventing microbial contamination in cell cultures is crucial. Implementing strict aseptic techniques, proper training, and laboratory controls are key to maintaining uncontaminated cell cultures and ensuring laboratory safety.

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Area of Science:

  • Cell Biology
  • Microbiology
  • Laboratory Science

Background:

  • Microbial contamination poses a significant threat to cell culture integrity and experimental validity.
  • Sources of contamination include personnel, the laboratory environment, other cell lines, and reagents.
  • Certain microbial infections can pose occupational health risks to laboratory personnel.

Purpose of the Study:

  • To outline strategies for preventing and eliminating microbial contamination in cell culture.
  • To emphasize the importance of containment and aseptic techniques for risk mitigation.
  • To provide guidance on managing widespread contamination events.

Main Methods:

  • Adherence to stringent aseptic techniques during all cell culture procedures.
  • Implementing robust containment strategies and personal protective equipment.
  • Regular monitoring and quality control of reagents and laboratory environment.
  • Proper training of staff in aseptic techniques and contamination management.
  • Quarantine protocols for new cell lines and reagents.
  • Thorough cleaning and maintenance of equipment and facilities.

Main Results:

  • Effective prevention significantly reduces the incidence of microbial contamination.
  • Prompt identification and remediation of contamination prevent widespread outbreaks.
  • A comprehensive approach involving personnel, environment, and procedures is essential.
  • Risk to laboratory workers is minimized through proper containment and aseptic practices.

Conclusions:

  • Proactive measures, including staff training, laboratory design, and strict adherence to aseptic techniques, are paramount for preventing cell culture contamination.
  • Effective management of contamination requires a systematic approach, including discarding affected materials, cleaning equipment, reviewing procedures, and isolating the issue.
  • Maintaining a high standard of quality control and implementing quarantine procedures are vital for long-term success in cell culture.