Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequential induction of nodulin gene expression in the developing pea nodule.

B Scheres1, F van Engelen, E van der Knaap

  • 1Department of Molecular Biology, Agricultural University, Wageningen, The Netherlands.

The Plant Cell
|August 1, 1990
PubMed
Summary

Early nodulin gene expression in pea root nodules is developmentally regulated. Different early nodulin mRNAs appear sequentially in specific root nodule zones, indicating distinct roles in infection and development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microsatellite fingerprinting in lettuce (Lactuca sativa L.) and wild relatives.

Plant cell reports·2019
Same author

Nodulin gene expression during soybean (Glycine max) nodule development.

Plant molecular biology·2013
Same author

cDNA cloning and developmental expression of pea nodulin genes.

Plant molecular biology·2013
Same author

Bleomycin resistance: a new dominant selectable marker for plant cell transformation.

Plant molecular biology·2013
Same author

Rhizobium nod genes are involved in the induction of two early nodulin genes in Vicia sativa root nodules.

Plant molecular biology·2013
Same author

Bacteroid-encoded proteins are secreted into the peribacteroid space by Rhizobium leguminosarum.

Plant molecular biology·2013

Area of Science:

  • Plant molecular biology
  • Legume symbiosis
  • Root nodule development

Background:

  • Early nodulins are key proteins in legume-Rhizobium symbiosis.
  • Understanding their temporal and spatial expression is crucial for deciphering nodule development.

Purpose of the Study:

  • To characterize early nodulin mRNAs in pea root nodules.
  • To investigate their expression patterns during nodule development.
  • To elucidate the roles of specific early nodulins.

Main Methods:

  • RNA transfer blot analysis to study mRNA expression over time.
  • In situ hybridization to determine transcript localization within root nodules.
  • Nucleotide and amino acid sequence analysis of cDNA clones.

Related Experiment Videos

Main Results:

  • Early nodulin mRNAs (ENOD12, ENOD5, ENOD3, ENOD14) exhibit distinct temporal expression patterns during nodule development.
  • Transcripts localize to specific zones within the root nodule central tissue.
  • ENOD5 is identified as an arabinogalactan-like protein involved in infection.
  • ENOD3 and ENOD14 are suggested to be metal-binding proteins.
  • Rhizobium induces ENOD12 and ENOD5 via distinct long- and short-distance signaling pathways.

Conclusions:

  • Early nodulin gene expression is tightly regulated in space and time during pea root nodule development.
  • Specific early nodulins play defined roles in infection and nodule maturation.
  • Distinct signaling mechanisms mediate the induction of infection-related early nodulin genes.