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Updated: May 18, 2026

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
Published on: April 18, 2016
Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections
Valérie Lalande1, Laurence Barrault, Sophie Wadel
1National Reference Laboratory for Clostridium Difficile, Université Pierre et Marie Curie, Site Saint-Antoine, 27 rue de Chaligny, 75012 Paris, France. valerie.lalande@sat.aphp.fr
Abstract:
A new assay (illumigene C. difficile; Meridian Bioscience), based on the original loop-mediated isothermal amplification (LAMP) assay, was evaluated with 472 unformed stools from patients suspected of Clostridium difficile infection. Compared to the toxigenic culture, the sensitivity, specificity, and positive and negative predictive values were 91.8, 99.1, 91.8, and 99.1% for the illumigene C. difficile assay and 69.4, 100, 100, and 96.6% for the cytotoxicity assay, respectively.
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