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Urinary Bladder01:23

Urinary Bladder

The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
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Voiding Cystourethrography (VCUG) and Cystography are specialized radiographic procedures used to examine the structure and function of the bladder and urethra.Voiding Cystourethrography (VCUG)A Voiding Cystourethrogram (VCUG) is a diagnostic imaging procedure that assesses the anatomy and function of the lower urinary tract. It focuses on the bladder, bladder neck, and urethra, helping detect abnormalities such as vesicoureteral reflux (VUR)—the backward or reverse flow of urine into the...
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Evaluation of Biomaterials for Bladder Augmentation using Cystometric Analyses in Various Rodent Models
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Published on: August 9, 2012

Bladder substitute reconstructed in a physiological pressure environment.

Sara Bouhout1, Robert Gauvin, Laure Gibot

  • 1Laboratoire d'Organogénèse Expérimentale de l'Université Laval, Génie tissulaire et régénération: LOEX, Centre de recherche FRSQ du Centre hospitalier affilié universitaire de Québec and Département de Chirurgie, Faculté de Médecine, Université Laval, Québec, QC, Canada. sara.ucergy@hotmail.fr

Journal of Pediatric Urology
|April 30, 2011
PubMed
Summary
This summary is machine-generated.

This study developed a dynamically cultured vesical equivalent (dcVE) using a bioreactor to mimic physiological bladder function. The dcVE shows improved urothelial profile and permeability, offering a promising autologous alternative for bladder reconstruction.

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Urology

Background:

  • Enterocystoplasty and current bioengineered substitutes for bladder reconstruction present significant complications.
  • Developing an autologous vesical equivalent (VE) with improved physiological properties is crucial.

Purpose of the Study:

  • To engineer a dynamically cultured VE (dcVE) in a bioreactor simulating physiological bladder dynamics.
  • To evaluate the structural and functional characteristics of the dcVE compared to a static VE.

Main Methods:

  • Fibroblasts and urothelial cells were cultured in 3D to form a VE.
  • The VE was dynamically cultured in a bioreactor with cyclic pressure (up to 15 cm H2O).
  • Histology, immunofluorescence, mechanical testing, and 14C-urea permeability assays were used for characterization.

Main Results:

  • Dynamic culture resulted in a urothelium resembling native bladder tissue.
  • The dcVE exhibited permeability comparable to native bladder.
  • Sufficient mechanical resistance for surgical handling was demonstrated.

Conclusions:

  • The dynamically cultured VE is a promising autologous option for regenerative medicine.
  • Its efficiency as a urea barrier may reduce inflammation and rejection.
  • This method advances bladder reconstruction alternatives.