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Assessing The Relationship Between Bladder Cancer Cell Contractile Phenotypes And Their Invasive Properties.

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Assessing The Relationship Between Bladder Cancer Cell Contractile Phenotypes And Their Invasive Properties.

Related Experiment Video

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Assessing the Relationship Between Bladder Cancer cell Contractile Phenotypes and their Invasive Properties.

Laurence Carignan^1,2,3,4,5, Reza Alavi^1,2,4,5, Martial Millet^1,2,4,5

¹Department of Molecular Biology, Medical Biochemistry and Pathology, Faculty of Medicine, Laval University, Québec, QC Canada.

Cellular and Molecular Bioengineering

|May 4, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

Bladder cancer cells with higher contractility are more invasive. Invasive bladder cancer cells exert greater traction forces and prefer stiffer environments, highlighting the link between cell mechanics and cancer aggressiveness.

Keywords:

Bladder cancer Cell contractility Durotaxis Invasiveness Mechanotransduction Substrate stiffness

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Area of Science:

Cancer Biology
Cell Mechanics
Biophysics

Background:

Increased cell contractility is a known hallmark of aggressive tumor cells in various cancers.
Bladder cancer (BLCA) is prevalent with a high recurrence rate, yet the link between cell contractility and its invasiveness is understudied.

Purpose of the Study:

To investigate the relationship between the contractile phenotype of cancer cells and their invasiveness in bladder cancer.
To establish a comparative baseline for mechanobiology investigations in BLCA cell lines.

Main Methods:

Assessed invasive potential of five BLCA cell lines using transwell and 3D spheroid invasion assays.
Measured cell contractility via traction force microscopy, 3D collagen gel compaction, and quantitative polarized light microscopy.

Quantified mechanotransduction through YAP nuclear translocation and analyzed stiffness-mediated cell migration on gradient substrates.

Main Results:

Identified T24 and MGH-U3 as invasive BLCA cell lines, while SW-780, RT4, and SW-1710 were non-invasive.
Invasive cell lines exhibited higher traction forces and increased YAP nuclear translocation on stiffer substrates compared to non-invasive lines.
Invasive cells migrated towards high stiffness, while non-invasive cells preferred low-to-mid stiffness on gradient substrates.

Conclusions:

Established a clear relationship between invasiveness and contractile phenotypes in BLCA cell lines.
Demonstrated that a single assay is insufficient to determine an aggressive phenotype.
Provided a foundational comparative dataset for future mechanobiology research in bladder cancer.