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Clarifying mammalian RISC assembly in vitro.

Grace S Tan1, Barry G Garchow, Xuhang Liu

  • 1Department of Medicine, Division of Rheumatology, University of Pennsylvania School of Medicine, Philadelphia, PA, USA.

BMC Molecular Biology
|May 3, 2011
PubMed
Summary

Argonaute 2 (Ago2) binds microRNA precursors (pre-miRNAs), forming active complexes. This study defines the minimal factors, Ago2 and Dicer, sufficient for pre-miRNA processing and RNA-induced silencing complex (RISC) loading.

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