Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Novel Y-STRs with elevated mutation rates further improve male relative differentiation.

Forensic science international. Genetics·2026

Same author

From cold case to conviction: How advanced DNA technologies such as mtDNA sequencing connected two brutal homicides.

Forensic science international. Genetics·2025

Same author

DNA methylation-based forensic framework for age prediction and body fluid identification using nanopore sequencing.

Forensic science international. Genetics·2025

Same author

Inter-laboratory evaluation of the VISAGE enhanced tool and models for age estimation from blood and buccal cells.

Forensic science international. Genetics·2025

Same author

mRNA profiling and donor association of mock casework samples: Results of a 3rd and 4th EDNAP collaborative exercise.

Forensic science international. Genetics·2025

Same author

Identifying a monozygotic twin brother as a donor of DNA in minimal, mixed forensic stains - A case example.

Forensic science international. Genetics·2025

Search research articles

Related Experiment Video

Updated: Jun 2, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

A protocol for direct and rapid multiplex PCR amplification on forensically relevant samples.

Saskia Verheij¹, Joyce Harteveld, Titia Sijen

¹National crime squad, Hoofdstraat 54, 3972 LB Driebergen, The Netherlands.

Forensic Science International. Genetics

|May 3, 2011

Summary

Forensic DNA typing is now faster, reducing the 10-12 hour process to 2-3 hours using rapid PCR. This enables quicker database searches for critical cases like national security threats.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Related Experiment Videos

Last Updated: Jun 2, 2026

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Traditional forensic DNA typing is a lengthy multi-step process, typically taking 10-12 hours.
Expedited DNA profiling is crucial for time-sensitive investigations, including national security, abductions, and suspect custody limitations.

Purpose of the Study:

To develop and validate a rapid direct polymerase chain reaction (PCR) approach for forensic DNA typing.
To significantly reduce the time required to obtain an interpretable short tandem repeat (STR) DNA profile suitable for database searching.

Main Methods:

Utilized a direct and rapid PCR method incorporating inhibitor-tolerant Phusion® Flash DNA polymerase and a modified allelic ladder.
Employed a quick PIKO® thermal cycler, ultra-thin walled tubes, and optimized interpretation guidelines for faster analysis.
Implemented mini-tapes for controlled sample input from swabs/fabrics, preserving the original sample for later analysis.

Main Results:

Reduced the total DNA profiling time to 2-3 hours post-DNA extraction.
Successfully obtained genotyping information for 10 STR markers plus the amelogenin (AMEL) marker.
Achieved high success rates for single-source samples like saliva, blood, semen, and hair roots, with results dependent on stain type and surface.

Conclusions:

The direct and rapid PCR method significantly accelerates forensic DNA profiling, enabling timely database searches.
This expedited process, integrated into a 'DNA-6h' service, enhances police investigations by rapidly providing crucial perpetrator information.
The method is effective for high-level DNA samples and preserves the original evidence for potential future analysis.