Related Experiment Video
Updated: Jun 2, 2026

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
Published on: March 14, 2025
Salinity-induced subcellular accumulation of H(2)O (2) in leaves of rice
Koji Yamane1, Mitsutaka Taniguchi, Hiroshi Miyake
1School of Agriculture, Kinki University, Nara, 631-8505, Japan.
Abstract:
The localization of salt-induced H(2)O(2) accumulation in the leaves of rice was examined using 3,3-diaminobenzidine and CeCl(3) staining at ultrastructure level. When the 3-week-old rice plants were affected by 100 mM NaCl for 14 days, the swelling of thylakoids and the destruction of thylakoid membranes were observed. H(2)O(2) accumulation was also observed in the chloroplast of the leaf treated with NaCl. The electron dense products of 3,3-diaminobenzidine and CeCl(3) were mainly observed especially around the swelling of thylakoids. H(2)O(2) accumulation and any ultrastructural changes were not observed in the chloroplasts under dark condition. Furthermore, treatment with ascorbic acid suppressed both H(2)O(2) accumulation and the changes in chloroplast ultrastructure. These results suggest that light-induced production of excess H(2)O(2) under salinity is responsible for the changes in chloroplast ultrastructure. H(2)O(2) accumulation was also observed in the mitochondria, peroxisomes, plasma membrane, and cell walls under light but not dark, suggesting that these organelles are also the source of H(2)O(2) and the production is light dependent under salinity.
More Related Videos
05:44Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay
Published on: November 25, 2022
14:20Measuring the Osmotic Water Permeability Coefficient (Pf) of Spherical Cells: Isolated Plant Protoplasts as an Example
Published on: October 8, 2014
Related Concept Videos
Responses to Salt Stress
Responses to Drought and Flooding
Tonicity in Plants
Tonicity in Plants
Tonicity
Tonicity describes the capacity of a cell to lose or gain water depending on the solute...
Adaptations that Reduce Water Loss
Osmosis